BioVision Technologies offers the following range of Sutter Instrument Company LED illumination products for brightfield and fluorescence microscopy applications:
The Lambda HPX represents the latest generation of liquid-cooled high-output LED light sources. Designed around a single 90W 4.25mm LED die, the Lambda HPX provides light output comparable to a 200W xenon arc lamp. A quiet, vibration-free liquid cooling head (pre-filled at the factory) allows maintaining the low junction temperatures required to reach the manufacturer’s projected bulb life of 60,000 hours. The HPX is expected to retain 95% of its original output at 5000 hours, and 80% after 10,000 hours. The lightweight precision-machined LED head is designed to mount directly on the epi port of a microscope using an included microscope adapter specified at time of order. This affords the maximum amount of light coupled directly to the scope, without the losses associated with a liquid light guide.
Because LEDs exhibit color shift with current change, the Lambda HPX is designed to dim the LED using either PWM or current control. PWM will be preferable for most applications, and allows the LED to run cooler. PWM switching is at 28KHz, high enough for use with most high speed cameras. For applications intolerant of pulsed output, dimming via current control is also available. PWM and current information are conveniently displayed on the front panel display, and are manually selectable via control knobs. Integral shuttering time is 10 microseconds to turn on and off.
PWM can also be controlled externally via analog input. TTL input and output allows for triggering from either software or directly from another device such as a camera or digital I/O board. The LED cable, and cooling lines are easily removed from the chassis via no-drip quick connectors. This allows for easy setup and routing of cables. Active temperature monitoring ensures that the LED life will be maximized.
Light output is in the visible spectrum from 430nm to 700nm. Both a cool white and a “tungsten” white LED is available. Special order units are available with 630nm, 530nm, 460nm, and 405nm wavelength specific LEDs. The Lambda HPX can also be combined with the Lambda TLED to create a two-channel system with any combination of wavelengths.
The Lambda HPX-L5 was created as a liquid light guide‐coupled version of the Lambda HPX high‐power LED light source. The liquid light guide allows for LED cooling via an internal heatsink with a whisper fan. This requires less maintenance than the liquid‐cooled direct mount version. Designed around a single 90W 3mm LED die, the Lambda HPX-L5 provides light output comparable to a 150W xenon arc lamp when using the same light guide. The Lambda HPX-L5 is optimized for coupling to an optional 5mm liquid light guide, and off-the-shelf microscope adapter. The HPX is expected to retain 95% of its original output at 5000 hours, and 80% after 10,000 hours.
Because LEDs exhibit color shift with current change, the Lambda HPX is designed to dim the LED using either PWM or current control. PWM will be preferable for most applications, and allows the LED to run cooler. PWM switching is at 28KHz, high enough for use with most high speed cameras. For applications intolerant of any pulsed output, dimming via current control is also available. PWM and Current information are conveniently displayed on the front panel display, and are manually selectable via control knobs. Integral shuttering time is 10 microseconds to turn on and off. TTL input and output allows for triggering from either software or directly from another device such as a camera or digital IO board. PWM can also be controlled externally via analog input. Active temperature monitoring ensures that the LED life will be maximized.
Light output is in the visible spectrum from 430nm to 700nm. Special order units are available with 630nm, 530nm, 460nm, and 405nm wavelength specific LEDs. The Lambda HPX-L5 can also be combined with our TLED to create a two-channel system with any combination of wavelengths.
The Lambda TLED and TLED+ are stand-alone LED light sources that can be used with the transmitted light path of a microscope or in other applications with similar requirements.
The basic system consists of an LED mounted on a special black-anodized aluminum heat sink and a controller. Both TLED and TLED+ controllers are powered by a rugged modular universal power supply. The controllers provide intensity control and on-off control via a toggle switch or TTL logic. The on-off time is < 25 µsecs when using TTL control. In addition to digital input control, the Lambda TLED+ has an analog input to modulate the LED intensity. The Lambda TLED and TLED+ are expected to have stable output that will last in excess of 50,000 hours.
The Lambda TLED and TLED+ can be ordered with a high-output white light LED making it a suitable light source for contrast methods including Phase and Differential Interference Contrast (DIC).
As an alternative to the standard white-light LED, we have several options available including an IR-LED which is suitable for experiments requiring IR-DIC. Please contact Sutter for details.
Our dual channel Lambda TLED+ option combines two high power LEDs into a single light path. The TLED-DC includes a 460nm LED for stimulation, while the second channel passes the phosphor emission from a white light LED. The white light channel can be used to access spectra between 510nm to 630nm and is suitable for a variety of applications. Both channels are driven by individual TLED+ controllers and can be triggered, also individually, by a TTL signal. Alternate wavelength configurations are available.
The LDB100F with 550 nanometer filter in the light path eliminates any background fluorescence. The LDB100F Brightfield LED illuminator provides all the advantages of LED illumination in a flexible package that can be fitted to most modern upright and inverted microscope systems.
The LDBUSBTTL adapter is a USB to TTL output converter that allows users to control simple devices such as TTL‐controlled shutters and transmitted LED light sources.