89 North Laser Diode Illuminators

BioVision Technologies offers the range of 89 North Laser Diode Illuminators.

89 North Logo

A Family of High‐performance Light Sources

The LDI is a multi‐line, solid‐state laser illuminator offering up to 1000 mW of output power via a multi‐mode fiber at the price of a low‐power LED light engine. With feedback‐controlled output stability and up to a 100:1 linear dynamic range, the LDI is the ideal light source for a wide range of applications including spinning disk confocal microscopy, structured illumination microscopy, FRAP, photoactivation/photoconversion, and PALM/STORM.

Scientific Applications

A LDI can be deployed across a wide range of scientific applications and techniques including:

  • Spinning Disk Confocal Microscopy
  • Super Resolution SIM Imaging
  • PALM/STORM
  • Optogenetics with a DLP or Multi‐port Illuminator
  • Photoactivation/Photoconversion/FRAP
  • FRAP with SLM or or Multi‐port Illuminator

Product Line Overview

  LDI is purchased with either 470 nm or 488 nm output   LDI is purchased with either 555 nm or 570 nm output  
  405 nm 445 nm 470 nm 488 nm 520 nm 528 nm 555 nm 577 nm 640 nm 730 nm
LDI 4-series 300 mW   1000 mW 1000 mW     1000 mW 700 mW 400 mW  
LDI 5-series 450 mW   1000 mW 1000 mW     1000 mW 700 mW 900 mW 850 mW
LDI 7-series 300 mW 1000 mW 1000 mW 1000 mW 500 mW 500 mW 1000 mW 700 mW 400 mW  
LDI‐NIR 450 mW 1000 mW 1000 mW 1000 mW 500 mW   1000 mW 700 mW 900 mW 850 mW
LDI PRIME 150 mW   N/A 800 mW     600 mW N/A 300 mW  

Features and Benefits

High Output Power

  • Shorter exposures
  • Faster imaging
  • Faster activation times in optogenetics and photoactivatin experiments
  • Faster bleaching in FRAP experiments

Feedback‐controlled Optical Stability

  • Quantitative imaging, ideally suited for ratiometric imaging
  • More repeatable optogenetics experiments

Ease of Use

  • Ability to turn power down when needed and maintain stability and quantitative imaging
  • Up to seven lines covers most of the standard fluorescence probes
  • No user alignment
  • Easy to use and maintain

LDI 4‐Series

The LDI‐4 laser diode illuminator is a user‐inspired version of the original LDI‐7. Many researchers were asking for a laser launch that covered only the 4 basic lines: DAPI, FITC, TRITC, Cy5. 89 North listened and we responded. The LDI‐4 has only the lines you asked for. While we reduced the number of lines inside the box, we made no compromises on the quality and performance you’ve come to expect from 89 North. This unit is also available with a 488 nm laser for FITC/GFP and with a 577 nm laser for TRITC/mCherry…or both!

Specifications
Source Type Laser diodes
Lifetime 20,000 hrs – two‐year warranty
Laser Lines (nm) 405 470 or 488 555 or 577 640
Continuous Wave Stability2 <2% <2% <2% <2%
Optical Power Min (mW)1 300 1000 1000 for 555 nm, 700 for 577 nm 400
Max Rise Time1 < 10 µs < 10 µs < 2 µs < 10 µs
Max On/Off Frequency (Hz)3 >1000 >1000 100 >1000
Output Options Optical fiber4
Control Options TTL on/off (> 2.3 V)
Analog intensity (0–5 V)
USB–DSP (virtual COM port) – SDK available upon request
Safety Interlocked housing
Safety interlock
Key interlock
IEC 60825‐compliant
Dimensions (L×W×D) 12.5×9.2×5.75”
318×234×146 mm
Weight ~9 lbs.
Operating Temperature 15–30 ̊C
Storage Temperature −18–50 ̊ C
Humidity < 80% non‐condensing
Voltage 90–220 V AC, 50–60 Hz
Fuse None
  1. Measured at 100% intensity, 23±2 °C,
  2. Standard Deviation relative to the mean power measurements, measured at 100% intensity, 23±2 °C.
  3. Measured at 100% intensity, 50% duty cycle.
  4. Recommended output fiber is 400 μm, 0.39 NA bifurcated fiber.
Features and Benefits
High Output Power Shorter exposures
Faster imaging
Faster activation times in optogenetics and photoactivation experiments
Faster bleaching times in FRAP experiments
Feedback‐controlled optical stability Quantitative imaging, ideally suited for ratiometric imaging
More repeatable optogenetics experiments
Up to 100:1 linear dynamic range Ability to turn power down when needed and maintain stability and quantitative imaging
4 laser lines across the spectrum Covers most of the standard fluorescence probes
No user alignment Ease of use and maintainability
TTL and Analog control Supports operation without software‐specific drivers
89 North LDI-4 Laser Diode Illuminator
89 North LDI-4 Laser Diode Illuminator

LDI 5‐Series

The LDI‐5 is the big sister of the LDI‐4 laser diode illuminator. Once again we listened to our users who asked for a pared down version of the LDI‐NIR. The LDI‐5 has increased power over her little sister and expands into the near infra‐red with the addition of a 730nm line. This unit is also available with a 488nm laser for FITC/GFP and with a 577nm laser for TRITC/mCherry…or both!

Specifications
Source Type Laser diodes
Lifetime 20,000 hrs – two‐year warranty
Laser Lines (nm) 405 470 or 488 555 or 577 640 730
Continuous Wave Stability2 <2% <2% <2% <2% <2%
Optical Power Min (mW)1 450 1000 1000 for 555 nm, 700 for 577 nm 900 850
Max Rise Time1 < 10 µs < 10 µs < 10 µs < 2 µs < 10 µs
Max On/Off Frequency (Hz)3 >1000 >1000 >1000 100 >1000
Output Options Optical fiber4
Control Options TTL on/off (> 2.3 V)
Analog intensity (0–5 V)
USB–DSP (virtual COM port) – SDK available upon request
Safety Interlocked housing
Safety interlock
Key interlock
IEC 60825‐compliant
Dimensions (L×W×D) 12.5×9.2×5.75”
318×234×146 mm
Weight ~9 lbs.
Operating Temperature 15–30 ̊C
Storage Temperature −18–50 ̊ C
Humidity < 80% non‐condensing
Voltage 90–220 V AC, 50–60 Hz
Fuse None
  1. Measured at 100% intensity, 23±2 °C,
  2. Standard Deviation relative to the mean power measurements, measured at 100% intensity, 23±2 °C.
  3. Measured at 100% intensity, 50% duty cycle.
  4. Recommended output fiber is 400 μm, 0.39 NA bifurcated fiber.
Features and Benefits
High Output Power Shorter exposures
Faster imaging
Faster activation times in optogenetics and photoactivation experiments
Faster bleaching times in FRAP experiments
Feedback‐controlled optical stability Quantitative imaging, ideally suited for ratiometric imaging
More repeatable optogenetics experiments
Up to 100:1 linear dynamic range Ability to turn power down when needed and maintain stability and quantitative imaging
5 laser lines across the spectrum Covers most of the standard fluorescence probes
No user alignment Ease of use and maintainability
TTL and Analog control Supports operation without software‐specific drivers
89 North LDI-5 Laser Diode Illuminator
89 North LDI-5 Laser Diode Illuminator

LDI 7‐Series

The LDI‐7 is a multi‐line, solid‐state laser illuminator offering up to 1000 mW of output power per laser line via a multi‐mode fiber at the price of lower power LED light engines. With feedback‐controlled output stability and up to a 100:1 linear dynamic range, the LDI is the ideal light source for a wide range of applications including spinning disk confocal microscopy, structured illumination microscopy, FRAP, photoactivation/photoconversion.

Specifications
Source Type Laser diodes
Lifetime 20,000 hrs – two‐year warranty
Laser Lines (nm) 405 445 470 or 488 520 528 555 or 577 640
Continuous Wave Stability2 <2% <2% <2% <2% <2% <2% <2%
Optical Power Min (mW)1 300 1000 1000 500 500 1000 for 555 nm, 700 for 577 nm 400
Max Rise Time1 < 10 µs < 10 µs < 10 µs < 10 µs < 10 µs < 2 µs < 10 µs
Max On/Off Frequency (Hz)3 >1000 >1000 >1000 >1000 >1000 100 >1000
Output Options Optical fiber4
Control Options TTL on/off (> 2.3 V)
Analog intensity (0–5 V)
USB–DSP (virtual COM port) – SDK available upon request
Safety Interlocked housing
Safety interlock
Key interlock
IEC 60825‐compliant
Dimensions (L×W×D) 12.5×9.2×5.75”
318×234×146 mm
Weight ~9 lbs.
Operating Temperature 15–30 ̊C
Storage Temperature −18–50 ̊ C
Humidity < 80% non‐condensing
Voltage 90–220 V AC, 50–60 Hz
Fuse None
  1. Measured at 100% intensity, 23±2 °C,
  2. Standard Deviation relative to the mean power measurements, measured at 100% intensity, 23±2 °C.
  3. Measured at 100% intensity, 50% duty cycle.
  4. Recommended output fiber is 400 μm, 0.39 NA bifurcated fiber.
Features and Benefits
High Output Power Shorter exposures
Faster imaging
Faster activation times in optogenetics and photoactivation experiments
Faster bleaching times in FRAP experiments
Feedback‐controlled optical stability Quantitative imaging, ideally suited for ratiometric imaging
More repeatable optogenetics experiments
Up to 100:1 linear dynamic range Ability to turn power down when needed and maintain stability and quantitative imaging
Up to seven laser lines Covers most of the standard fluorescence probes
No user alignment Ease of use and maintainability
TTL and Analog control Supports operation without software‐specific drivers
89 North LDI-7 Laser Diode Illuminator
89 North LDI‐7 Laser Diode Illuminator

LDI‐NIR

The LDI‐NIR is the same great multi‐line, solid‐state laser illuminator as the LDI‐7, but offers more power and extends the range into the NIR. This affordable multi‐mode laser launch will change how you imagine laser illumination.

Specifications
Source Type Laser diodes
Lifetime 20,000 hrs – two‐year warranty
Laser Lines (nm) 405 445 470 or 488 520 555 or 577 640 730
Continuous Wave Stability2 <2% <2% <2% <2% <2% <2% <2%
Optical Power Min (mW)1 450 1000 1000 500 1000 for 555 nm, 700 for 577 nm 900 850
Max Rise Time1 < 10 µs < 10 µs < 10 µs < 10 µs < 2 µs < 10 µs < 10 µs
Max On/Off Frequency (Hz)3 >1000 >1000 >1000 >1000 >1000 100 >1000
Output Options Optical fiber4
Control Options TTL on/off (> 2.3 V)
Analog intensity (0–5 V)
USB–DSP (virtual COM port) – SDK available upon request
Safety Interlocked housing
Safety interlock
Key interlock
IEC 60825‐compliant
Dimensions (L×W×D) 12.5×9.2×5.75”
318×234×146 mm
Weight ~9 lbs.
Operating Temperature 15–30 ̊C
Storage Temperature −18–50 ̊ C
Humidity < 80% non‐condensing
Voltage 90–220 V AC, 50–60 Hz
Fuse None
  1. Measured at 100% intensity, 23±2 °C,
  2. Standard Deviation relative to the mean power measurements, measured at 100% intensity, 23±2 °C.
  3. Measured at 100% intensity, 50% duty cycle.
  4. Recommended output fiber is 400 μm, 0.39 NA bifurcated fiber.
Features and Benefits
High Output Power Shorter exposures
Faster imaging
Faster activation times in optogenetics and photoactivation experiments
Faster bleaching times in FRAP experiments
Feedback‐controlled optical stability Quantitative imaging, ideally suited for ratiometric imaging
More repeatable optogenetics experiments
Up to 100:1 linear dynamic range Ability to turn power down when needed and maintain stability and quantitative imaging
Up to seven laser lines Covers most of the standard fluorescence probes (405 nm, 445 nm, 470 nm, 520 nm, 555 nm, 640 nm, 730 nm)
No user alignment Ease of use and maintainability
TTL and Analog control Supports operation without software‐specific drivers
89 North LDI-NIR Laser Diode Illuminator
89 North LDI‐NIR Laser Diode Illuminator

LDI‐PRIME

The LDI‐PRIME laser diode illuminator was designed specifically for those researchers whose work demands the 488 nm laser. As this is a premium laser line, we combined it with a collection of three other lasers to cover the visible spectrum as simply as possible to provide essential power and performance at an affordable price. To keep it simple we also combined all the lasers into a single 400 µm fiber optic output.

Specifications
Source Type Laser diodes
Lifetime 20,000 hrs – two‐year warranty
Laser Lines (nm) 405 488 555 640
Continuous Wave Stability2 <2% <2% <2% <2%
Optical Power Min (mW)1 150 800 600 300
Max Rise Time1 < 10 µs < 10 µs < 10 µs < 10 µs
Max On/Off Frequency (Hz)3 >1000 >1000 >1000 >1000
Output Options Optical fiber4
Control Options TTL on/off (> 2.3 V)
Analog intensity (0–5 V)
USB–DSP (virtual COM port) – SDK available upon request
Safety Interlocked housing
Safety interlock
Key interlock
IEC 60825‐compliant
Dimensions (L×W×D) 12.5×9.2×5.75”
318×234×146 mm
Weight ~9 lbs.
Operating Temperature 15–30 ̊C
Storage Temperature −18–50 ̊ C
Humidity < 80% non‐condensing
Voltage 90–220 V AC, 50–60 Hz
Fuse None
  1. Measured at 100% intensity, 23±2 °C,
  2. Standard Deviation relative to the mean power measurements, measured at 100% intensity, 23±2 °C.
  3. Measured at 100% intensity, 50% duty cycle.
  4. Recommended output fiber is 400 μm, 0.39 NA bifurcated fiber.
Features and Benefits
High Output Power Shorter exposures
Faster imaging
Faster activation times in optogenetics and photoactivation experiments
Faster bleaching times in FRAP experiments
Feedback‐controlled optical stability Quantitative imaging, ideally suited for ratiometric imaging
More repeatable optogenetics experiments
Up to 100:1 linear dynamic range Ability to turn power down when needed and maintain stability and quantitative imaging
4 laser lines Covers most of the standard fluorescence probes (405nm, 488 nm, 555 nm, 640 nm)
No user alignment Ease of use and maintainability
TTL and Analog control Supports operation without software‐specific drivers
89 North LDI-PRIME Laser Diode Illuminator
89 North LDI‐PRIME Laser Diode Illuminator

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