Confocal imaging system from Crest Optics
The X-Light V2tp is a unique system for spinning disk confocal imaging. It features unparalleled versatility:
The X-Light V2tp Confocal Imager is a full-spectrum spinning disk confocal imager that attaches to most major models of inverted or upright fluorescence microscope. It is designed to utilize the latest imaging technologies and is ideal for live cell imaging in biological applications.
The X-Light V2tp Confocal Imager allows imaging at the extremes of performance of a current‐generation scientific camera, and it provides for objectives used when imaging with a camera with a smaller detector. It takes advantage of laser launches and LED light sources.
The result is a flexible confocal add‐on for existing microscopes that brings the latest commercialized technologies to microscopy. The X-Light V2tp Confocal Imager is an extraordinary combination of capability and price.
A rapid motorized Disk In/DiskOut control provides access to widefield and confocal imaging modes. Switching between modes is automated by imaging software.
A proprietary disk pattern design provides high confocal resolution, improved out‐of‐focus rejection and higher ratio of signal‐to‐noise. The result is < 800 nm confocal resolution with 60x 1.4 N.A. oil immersion objective.
Disks are available with pinholes sized for best results from different objectives:
Disks are available to best suit particular camera setups.
The disk rotates at 15,000 revolutions per minute, providing excitation and imaging at speeds of current cameras.
Custom relay lenses mitigate aberrations of of a large field of view.
The X-LIGHT V2tp addresses difficulties of both initial set-up and continuing maintenance of a confocal imager.
The VCS (“Video Confocal Super‐resolution”) module is an add‐on for the X-Light V2.
CrestOptics VCS enhances the 3‐D resolution of the standard light microscope. It performs a two‐dimensional scan of the sample with a pinhole pattern and applies multiple algorithms to process the the emission, producing the image. The VCS technology is patented.
To speed the extensive calculation required, the VCS module uses CUDA technology from NVidia. The result is very fast processing.
The addition of the VCS module to the X-Light V2 provides a confocal imager with three modes of operation: Widefield Mode, Confocal Mode, and Super-resolution Mode.
VCS (“Video Confocal Super‐resolution”) is based on narrow-field illumination using scanning patterns and wide-field collection of raw images.
Detection algorithms already developed are able to super-resolve 3‐D structures in both compact and sparse specimens.
Although other techniques proposed and industrially developed mainly dedicate their efforts in extracting information from a relatively low spatial frequency range, VCM detection methods harness non-linear calculations exploiting the tops more than the belly of the raw signal intensity, collected as a function of illumination and detection positional parameters.
The figure below shows the schematic diagram for a basic VCS microscopy system. In this set‐up, the light source is focused onto a specified pattern that is optically conjugated to the microscope conjugate focal plane through a relay system. The mask is moved in the optical path with an X‐Y piezo motor system at each camera acquisition. The emission is collected widefield, and extensive processing performed to produce the final image.
The figure below shows a typical illumination pattern projected onto a sample.