Tracking Single Molecules and Vesicles in Cells with Oxford Nanoimaging Nanoimager

TRP channel proteins tagged with mCherry and expressed in HEK cells

We can understand the dynamics, trajectories and interaction of single molecules and vesicles in cells. This video shows TRP channel proteins tagged with mCherry and expressed in HEK cells. They are transported to the plasma membrane in vesicles, which are visible as the highly dynamic bright fluorescent spots moving throughout the cells.

Vesicle Trajectories
Trajectories followed by vesicles
RNA polymerase enzymes tracked using Oxford Nanoimaging Nanoimager
Single RNA polymerase enzymes tagged with PA-mCherry

One of the images above shows the trajectories followed by the vesicles as mapped out using Nanoimager tracking software. The other image shows single RNA polymerase enzymes tagged with PA-mCherry and tracked using single-particle tracking photoactivated localization microscopy.