Confocal Microscopy

The confocal microscope excites the samples with a focused spot of light. This spot is rapidly moved through the lateral axes of the specimen to excite the fluorescent components, which may be done with a laser scanning module. Spinning disk confocal excites and collects the emission from multiple points simultaneously, offering improved speed of imaging by multiplexing. The emitted light is directed though a pinhole aperture and out-of-focus light is rejected, resolving issues from the widefield microscope.