Excelitas Technologies LED Light Sources
BioVision Technologies offers the following LED microscope illumination products from Excelitas Technologies Corporation:
X‐Cite® TURBO with LaserLED Hybrid Drive™
Powerful, multi‐wavelength LED illumination for all your Fluorophores
The X‐Cite TURBO is a powerful, six‐wavelength LaserLED Hybrid source for fluorescence excitation applications.
Providing maximum excitation for up to six fluorophores using Excelitas Technologies’ patent‐pending LaserLED Hybrid Drive™, X‐Cite TURBO offers a highly versatile solution for routine to advanced imaging applications.
With manual, PC, TTL and analog control, as well as compatibility with existing imaging software drivers, X‐Cite TURBO includes convenient control options for any instrument configuration.
With six high‐power wavelengths, researchers have the flexibility to add additional fluorophores for live or fixed cell imaging to their imaging arsenal.
Features and Benefits
| Feature | Benefit |
| LaserLED Hybrid Drive™ | Maximum output power. Operate six wavelengths individually, simultaneously or in any combination with no “channel sharing” or delays to change internal filters. |
| Excitation Filter Holders | Precision. Fine‐tune excitation spectra to your requirements. |
| Fast‐switching | Excitation uniformity. Direct light into instrument with a light guide style that best suits the application. Main unit can be remotely located if space is constrained. |
| Multiple Control Options | Simplicity. Operate with USB, TTL, analog or a combination. |
| Optimized Thermal Management | Reliability. Maximize LED output, lifetime, and stability. |
| X‐Cite Coupling Optics – optional | Performance. Maximize optical output and uniformity with the highest performing coupling optics available. |
Control Options
| Control Mode | Available Control |
| Manual Control – SpeedDIAL | ON/OFF |
| Speed‐sensitive intensity control | |
| Left‐ and right‐handed control option | |
| USB | ON/OFF |
| Intensity control | |
| Compatible with X‐Cite XLED1 third‐party software drivers | |
| TTL | ON/OFF |
| Analog Voltage | 1-5 V |
X‐Cite® XLED1
Advanced LED light source for fluorescence excitation
The X‐Cite® XLED1 is a high‐power LED microscope light source designed to optimize fluorophore excitation with unmatched field uniformity at the specimen.
Its unique plug-and-play modularity allows the system to evolve alongside changing research applications, with easily interchangeable LED modules.
With unprecedented wavelength switching speeds to capture fast cell dynamics and advanced triggering options to extend live-cell imaging experiments, the X‐Cite® XLED1 represents the industry's next generation of fluorescence LED illumination.
Features and Benefits
| Feature | Benefit |
| Maximum power and individual LED control | Use up to four high power LED modules with fine excitation control while balancing illumination intensity between channels and protecting specimens against photodamage |
| Unmatched field uniformity at the specimen | X-Cite microscope adaptors lead the industry in field uniformity without the need for alignment, while saving time on maintenance and ensuring peace-of-mind in experimental results. |
| Adaptable plug-and-play modularity | Each LED module and associated optical components can be quickly swapped in the field for another wavelength depending on the needs of your application. |
| Rapid wavelength switching to capture fast cell dynamics | Each LED module is designed to integrate and easily interchange individual excitation filters, allowing accelerated wavelength switching beyond the scope of motorized filter wheels. |
| Flexible triggering for sequential or simultaneous imaging | Triggering sequences can be combined with a high degree of control over individual LED intensity to excite and image multiple fluorophores when examining very fast moving specimens or for live-cell ratio imaging. |
| Live-cell mode to limit photobleaching and cellular damage | Researchers can extend the time frame of their live-cell imaging experiments by reducing the degree of free radical formation caused by the continuous illumination of fluorescent proteins. |
Bundled Systems and Custom Configurations
To simplify system selection, the XLED1 offers three bundled configurations as standard options that are designed to address primary fluorescence applications. These bundles provide a complete system solution with the following components included:
- X-Cite® XLED1 base unit and GUI software
- Four LED modules and appropriate dichroics
- One liquid light guide and microscope adaptor
- Set of four excitation filter holders
Researchers also have the flexibility to tailor an XLED1 system as necessary to meet the needs of their application.
| LED Module | Excitation Range-of-Use | Inclusion in Bundled System | ||
|---|---|---|---|---|
| Live Cell | Fixed Cell #1 | Fixed Cell #2 | ||
| UVA | 360-380 nm | ● | ||
| UVX | 375-400 nm | ● | ||
| UVV | 390-415 nm | |||
| BLX | 430-465 nm | ● | ||
| BDX | 450-495 nm | ● | ● | ● |
| BGX | 505-545 nm | ● | ||
| GYX | 540-600 nm | ● | ● | ● |
| RLX | 615-655 nm | ● | ● | |
| RDX | 660-675 nm | |||
| IRX | 710-750 nm | |||
Excitation Filters
The XLED1 may need filters installed in the module to further narrow the excitation spectra for your fluorophore of choice in order to perform fast imaging without crosstalk. BioVision Technologies offers filter sets from Chroma Technology and Semrock. Please contact your local BioVision Technologies representative for further information.
X‐Cite® 120LED
High‐Power, Broad-Spectrum LED solution for Fluorescence Microscopy
The X‐Cite® 120LED delivers LED illumination with no compromise. It offers superior optical power and exceptional field uniformity at the specimen level with the broadest spectrum of fluorescence excitation. Suited to multi-day time-lapse experiments, with LEDs guaranteed for 25,000 hours and no lamps or modules to replace, X‐Cite 120LED offers simplicity and convenience, allowing the researcher to focus on experiments instead of equipment maintenance.
An innovative thermal management design allows direct coupling to the microscope, providing maximum power without adding mechanical vibration. An electronic shutter provides fast, sub‐millisecond operation enabling extreme precision in vibration-sensitive imaging experiments.
The X‐Cite 120LED provides for control via leading imaging software such as MetaMorph®, MetaFluor® or iVision‐Mac™, or via TTL signaling, or manually via the speedDIAL. Instant LED on/off capability minimizes photobleaching and phototoxicity while offering ultra-fast software control and TTL triggering.
With zero mercury disposal and low energy consumption, the X‐Cite® 120LED is a highly efficient, environmentally‐friendly solution that can adapt to any system configuration including microscopes from all major manufacturers.
Features and Benefits
| Feature | Benefit |
|---|---|
| High Power LEDs | Efficient fluorophore excitation |
| Broad Spectrum | Excitation of a variety of fluorophores |
| Excellent Field Uniformity | Reliable image data |
| Instant ON/OFF (electronic shuttering) | No warm‐up, no vibration from mechanical shuttering |
| Several Control Options | Manual, USB, TTL, third‐party software |
| Direct Coupling | Maximum light transmission to sample |
| LED Technology | Long‐life, stable, reliable, maintenance‐free |
| Unmatched Thermal Management | Vibration‐free imaging |
| Simple Operation | Fingertip control, no training required |
| Less Toxic Waste | No mercury disposal issues |
X‐Cite® 110LED
The X‐Cite® 110LED is a compact, white light LED light source for fluorescence imaging applications. Providing excitation for DAPI, GFP, mCherry and Cy5 routine imaging, the X-Cite 110LED offers exceptional field uniformity through liquid light guide delivery. With manual, PC and TTL control, the X-Cite 110LED has LEDs guaranteed for 25,000 hours with no lamps or modules to replace.
The X‐Cite 110LED provides for control via leading imaging software such as MetaMorph®, MetaFluor® or iVision‐Mac™, or via TTL signaling, or manually via the speedDIAL. Instant LED on/off capability minimizes photobleaching and phototoxicity while offering ultra-fast software control and TTL triggering.
With zero mercury disposal and low energy consumption, the X‐Cite® 110LED is a highly efficient, environmentally‐friendly solution that can adapt to any system configuration including microscopes from all major manufacturers.
Features and Benefits
| Feature | Benefit |
| LED Technology | Mercury-free, higher efficiency, stability, instant ON/OFF and long lifetime |
| White Light Excitation | Excitation of commonly used fluorophores such as DAPI, GFP/FITC, mCherry/Texas Red, and Cy5 |
| Light Guide Delivery | Flexibility in light delivery as well as unit location |
| Multiple Control Options | Simple operation through USB, TTL or speedDIAL |
| Small Footprint | Minimal space requirement; less bench top real estate used |
| Optimized Thermal Management | Maximum LED output, lifetime, stability and reliability |
| X-Cite Coupling Optics (optional) | Efficient optical output and uniformity with the highest performing coupling optics available |
Control Options
| Manual Control – speedDIAL | ON/OFF |
| Speed‐sensitive intensity control | |
| One favorite intensity setting | |
| Left‐ and right‐handed control option | |
| USB | ON/OFF |
| Intensity control | |
| Compatible with drivers for X-Cite 120PC, X-Cite exacte, X-Cite 120LED | |
| TTL | ON/OFF |
X‐Cite® 120LEDmini
Compact and Simple‐to‐Use Broadband Illumination
The X-Cite® 120LEDmini is a compact and simple to use white‐light LED light source for fluorescence imaging applications. Through direct coupling, X-Cite 120LEDmini delivers superior LED illumination and exceptional field uniformity at the specimen level with the broadest spectrum of fluorescence excitation for routinely used fluorophores. Additionally, with manual, PC and TTL control, X-Cite 120LEDmini provides convenient control options for any instrument configuration, from basic to fully automated operation. With LEDs guaranteed for 20,000 hours, X-Cite 120LEDmini is virtually maintenance‐free with no lamp or light guide components to replace, and requires minimum space attributed to its compact size.
An innovative thermal management design allows direct coupling to the microscope, providing maximum power without adding mechanical vibration. An electronic shutter provides fast, sub‐millisecond operation enabling extreme precision in vibration-sensitive imaging experiments.
The X‐Cite 120LED provides for control via leading imaging software such as MetaMorph®, MetaFluor® or iVision‐Mac™, or via TTL signaling, or manually via the speedDIAL. Instant LED on/off capability minimizes photobleaching and phototoxicity while offering ultra-fast software control and TTL triggering.
With zero mercury disposal and low energy consumption, the X‐Cite® 120LED is a highly efficient, environmentally‐friendly solution that can adapt to any system configuration including microscopes from all major manufacturers.
Features and Benefits
| Feature | Benefit |
|---|---|
| LED Technology | Efficiency, stability, instant ON/OFF and long lifetime |
| White Light Excitation | Excitation of commonly used fluorophores such as DAPI, CFP, GFP/FITC, YFP, mCherry/Texas Red, and Cy5 |
| Direct Coupled | High Power excitation |
| Multiple Control Options | Simple operation through USB, TTL or SpeedDIAL |
| Small Footprint | Minimal space requirement; less bench top real estate used |
| Optimized Thermal Management | Maximum LED output, lifetime, stability and reliability |
| X-Cite Coupling Optics | Efficient optical output and uniformity with highest performing coupling optics |
