CoolLED Light Sources

BioVision Technologies offers the following microscope illumination products from CoolLED Ltd.:

CoolLED Logo

pE-4000

The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy. The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, giving it the broadest spectrum of illumination available.

For consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system.

For total control over the excitation source to improve imaging, the pE-4000 “Advanced” mode provides selection and control of individual wavelengths settings.

Features and Benefits

Feature Benefit
Broadest LED spectrum available: 365-770nm Matches LED peaks to existing microscope filters
Four channel wavelength-grouping design Matches with all commercially available dual, triple, and quad filters
Individual control of selected LED wavelengths Less background, no filter wheel or shutters
Integrated with many imaging software packages Can be incorporated into all experimental parameters
Instant on/off No shutters required, no warm up or cool down
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time

Features for Multi-User Facilities and Core Facilities

Feature Benefit
Feature Benefit
Presets Lab manager can match white spectrum to existing filter cubes
Higher‐contrast images From matched white spectrum
Simple installation and use  
No training required  

Features for Advanced Research

Feature Benefit
Individual LED wavelength selection Matches LED peaks to existing microscope filters
Rapid switching between LED wavelengths Enables capture of high‐speed events
TTL and USB interfaces Flexible integration with imaging software
Allows installation of excitation filters in optical path Provides controlled switching with no moving parts
Analog voltage inputs for intensity control High-speed switching
Optical Feedback Provides for applications requiring higher stability
Internal function generator For electophysiology and optogenetics applications

Specifications

Power Values
Channel Wavelength Power Full Width at Half Maximum
Channel 1 365 nm 30 mW 10 nm
385 nm 101 mW 10 nm
405 nm 188 mW 18 nm
435 nm 170 mW 17 nm
Channel 2 460 nm 241 mW 20 nm
470 nm 167 mW 20 nm
490 nm 87 mW 31 nm
500 nm 58 mW 29 nm
Channel 3 525 nm 35 mW 31 nm
550 nm 245 mW 85 nm
580 nm 236 mW 85 nm
595 nm 37 mW 15 nm
Channel 4 635 nm 136 mW 16 nm
660 nm 193 mW 19 nm
740 nm 76 mW 39 nm
770 nm 18 mW 53 nm

Powers measured at sample plane of research grade microscope using 100% mirror in cube and 10× objectives. (Figures are typical values).

Note: wavelength settings 550nm and 580nm use single broad, high intensity peak

Other Specifications
Parameter Specification
Power Requirements 110–240V AC. 50/60Hz, 2.5A
Power Consumption Standby (no LEDs active) Maximum 7W
Single‐wavelength Operation Maximum 41W
Dual‐wavelength Operation Maximum 75W
Triple‐wavelength Operation Maximum 93W
Quad‐wavelength Operation Maximum 112W
Dimensions, W×D×H Light Source 150×220×260 mm
Control Pod 154×135×40 mm
Power Supply 164×64×35 mm
Expansion Box 151×18×95 mm
Weight Light Source 3.5 kg
Control Pod 0.95 kg
Power Supply 0.58 kg
Expansion Box 0.34 kg
Environment and Safety Mercury‐free
No bulb replacement needed
Long lifetime (25,000 operating hours)
No special disposal regulations or issues
Reduced risk of eye damage
Energy‐efficient: 80% less power than conventional illuminators
Quiet operation

Control

Mode Description
Manual Dual‐function remote manual control pod for White mode or Advanced mode
Remote Via USB for independent on/off and intensity control of each channel. Triggering speed approx. 1 ms
Via 4 TTL inputs for independent on/off control of each channel. Triggering speed <20 µs
Via single TTL for on/off control of manual or software‐selected channels
Via 4 analog inputs 0‐10V, 0‐300kHz for dynamic control of intensity from external analog signals
Synch Out 4 TTL outputs for each channel – active high
1 TTL output for any channel – active high
Programmable Interface 4 TTL outputs for on/off control of peripherals (transmitted illuminators, stages etc.).
Function Generator Internally‐generated sine, pulse and ramps for each channel, programmed via control pod.
Connectivity Connection to PC is USB 2.0 Type B.
All other TTL and Analogue inputs/outputs via DB‐25 female connector. Optional rear‐mounting pE-Expansion Box available for BNC connectivity
Imaging Software Supported by leading imaging software including MetaMorph, MetaFluor, MetaVue, iVision‐Mac
CoolLED pE-4000 light source and control pod
CoolLED pE-4000 light source and control pod

pE-300ultra

CoolLED’s pE-300ultra is a fluorescence microscopy light source which offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains. Users have access to both microsecond switching via multiple TTL inputs and the ability to mount inline excitation filters. This, when paired with today’s high performance multi band filter sets, facilitates imaging traditionally done via a white light source and a filter wheel, with all the benefits of LED.

The pE-300ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300lite and the pE-300white, the System offers precise control over wavelength intensity and shuttering. Until now these benefits have only been accessible to users of high end, highly flexible illuminators such as the CoolLED pE-4000.

The pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.

The pE-300ultra includes CoolLED’s “Sequence Runner” multiple‐channel excitation mode. Users can define the order of their fluorophore capture using their pE-300ultra Control Pod, then the pE-300ultra light source can accept a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the light source. This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.

Features and Benefits

Feature Benefit
Feature Benefit
Clean bright illumination across the spectrum Excites common fluorescent stains
Order for existing single and multi-band filter sets No need to buy new filters
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use No alignment, a once only adjustment
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time
Individual channel triggering via TTL Triggering time of microseconds
Sequence Runner Sequenced excitation from a single TTL output

Specifications

Parameter Specification
DAPI Excitation Options 365 nm waveband configuration For microscope with typical single‐band filter sets
400 nm waveband configuration For microscope with typical multi‐band filters
Power Requirements 110–240V AC. 50/60Hz, 2A
Power Consumption Standby (no LEDs active) Maximum 2W
One‐band (GYR) at 100% intensity Maximum 20W
Two‐band (BLU & GYR) at 100% intensity Maximum 38W
White (all three bands) at 100% intensity Maximum 46W
Dimensions, W×D×H Light Source 77×186×162 mm
Control Pod 88×125×37 mm
Power Supply 167×67×35 mm
Universal Collimator 44×86×44 mm
Weight Light Source 1.4 kg
Control Pod 0.32 kg
Power Supply 0.62 kg
Universal Collimator 0.17 kg
Environment and Safety Mercury‐free
No bulb replacement needed
Long lifetime (25,000 operating hours)
No special disposal regulations or issues
Reduced risk of eye damage
Energy‐efficient: 80% less power than conventional illuminators
Quiet operation
Warranty System 36 months
LEDs 36 months

Control

Mode Description
Manual Manual control for instant on/off and intensity control in 1% steps from 0‐100%. Individual bands can be selected/deselected and controlled independently as desired.
Remote Via USB for independent on/off and intensity control of each channel.
Via global and individual channel TTL for on/off control of selected channels, using a BNC on the light source. Triggering speed <20 µs at full power
Sequence Runner Single TTL input to step through user‐defined sequence. Triggering speed <20 µs at full power
Connectivity Connection to PC is USB 2.0 Type B.
Imaging Software Supported by leading imaging software including MetaMorph, MetaFluor, MetaVue, iVision‐Mac

pE-300white

CoolLED’s pE-300white offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains. The pE-300white Light Source can be fitted directly to the microscope or specified with a liquid light guide. The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators. The system is perfect for hospital and regular research laboratory environments.

The pE-300white is a compact and simple to use LED illumination unit. It provides a broad spectrum of illumination, covering the excitation bands of common fluorescent stains, from the UV (DAPI excitation) to the Red region (Cy5 excitation). Manual operation is by a remote control pod with instant on/off and intensity control from 0 to 100 percent. Remote control is available via USB or TTL trigger.

Features and Benefits

Feature Benefit
Feature Benefit
Clean bright illumination across the spectrum Excites common fluorescent stains
Order for existing single and multi-band filter sets No need to buy new filters
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use No alignment, a once only adjustment
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time

Specifications

Parameter Specification
DAPI Excitation Options 365 nm waveband configuration For microscope with typical single‐band filter sets
400 nm waveband configuration For microscope with typical multi‐band filters
Power Requirements 110–240V AC. 50/60Hz, 2A
Power Consumption Standby (no LEDs active) Maximum 2W
One‐band (GYR) at 100% intensity Maximum 20W
Two‐band (BLU & GYR) at 100% intensity Maximum 38W
White (all three bands) at 100% intensity Maximum 46W
Dimensions, W×D×H Light Source 77×186×162 mm
Control Pod 88×125×37 mm
Power Supply 167×67×35 mm
Universal Collimator 44×86×44 mm
Weight Light Source 1.4 kg
Control Pod 0.32 kg
Power Supply 0.62 kg
Universal Collimator 0.17 kg
Environment and Safety Mercury‐free
No bulb replacement needed
Long lifetime (25,000 operating hours)
No special disposal regulations or issues
Reduced risk of eye damage
Energy‐efficient: 80% less power than conventional illuminators
Quiet operation
Warranty System 36 months
LEDs 36 months

Control

Mode Description
Manual Manual control for instant on/off and intensity control in 1% steps from 0‐100%. Individual bands can be selected/deselected and controlled independently as desired.
Remote Via USB for independent on/off and intensity control of each channel.
Via single TTL for on/off control of selected channels, using a single BNC on the light source. Triggering speed <20 µs
Connectivity Connection to PC is USB 2.0 Type B.
Imaging Software Supported by leading imaging software including MetaMorph, MetaFluor, MetaVue, iVision‐Mac

pE-300lite

The pE-300lite is a simple white light LED illumination unit. It provides a broad spectrum of illumination, covering the excitation bands of common fluorescent stains. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. With the new enhanced pE-300lite, this simple white light, at full power uses just 46 Watts.

This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology. Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

The system comprises a pE-300lite Light Source, control pod, and power supply. It can be ordered with a microscope adaptor for direct‐fit configuration or with a 3 mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor are available for use with a liquid light guide.

As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre‐aligning samples before scanning commences. In this way, photobleaching of the sample can be minimized when LED illumination is used.

With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300lite can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

Features and Benefits

Feature Benefit
Simplicity of Purchase
Clean bright illumination across the spectrum Works with everyday fluorophores/stains
Affordability Purchase through lab consumables budget
Broad Spectrum Covers UV (DAPI) to Red region (Cy5)
Order for existing single and multi-band filter sets No need to buy new filters
Simplicity of Installation
Simple to fit Direct fit to the microscope in seconds
Wide range of microscope adaptors Fits most microscopes
No alignment A once‐only adjustment
Simplicity of Use
Instant on/off No shutters required, no warm up or cool down
Stable and repeatable Reliable and consistent results
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time

Specifications

Parameter Specification
DAPI Excitation Options 365 nm waveband configuration For microscope with typical single‐band filter sets
400 nm waveband configuration For microscope with typical multi‐band filters
Power Requirements 110–240V AC. 50/60Hz, 1.4A
Power Consumption Standby 2W
Full White Intensity 46W
Dimensions, W×D×H Light Source 77×186×162 mm
Control Pod 88×125×37 mm
Power Supply 167×67×35 mm
Universal Collimator 44×86×44 mm
Weight Light Source 1.4 kg
Control Pod 0.32 kg
Power Supply 0.62 kg
Universal Collimator 0.17 kg
Environment and Safety Mercury‐free
No bulb replacement needed
Long lifetime (25,000 operating hours)
No special disposal regulations or issues
Reduced risk of eye damage
Energy‐efficient: 80% less power than conventional illuminators
Quiet operation
Warranty System 36 months
LEDs 36 months

Control

Mode Description
Manual Manual control for instant on/off and intensity control in 1% steps from 0‐100.
CoolLED pE-300ultra light source and control pod
CoolLED pE-300ultra light source and control pod

pE-100wht

The pE-100wht is a powerful white LED illumination system designed to replace a 100W halogen lamp. Histologists and cytologists who are familiar with results from a conventional halogen lamp can immediately make accurate and reliable diagnosis using the pE-100wht as colors will appear the same. Unlike that of a Halogen lamp, the color balance of the pE-100wht illumination system does not vary with intensity, removing the need to make any adjustment. The many additional benefits which LEDs offer—mercury-free operation, instant on/off, control and repeatability—mean that laboratories using halogen lamps will find that the move to the pE-100wht is obvious and simple.

The pE-100wht is a compact and simple-to-use LED illumination system for transmitted light applications and comprises a pE-100wht LED light source, control pod, and power supply. The user is given instant on/off and intensity control in one‐percent steps from the manual control pod which can be positioned close at hand. Remote control is available via a TTL trigger.

The system uses a new LED technology which has a spectrum closely matching halogen. Previous LED technology offered illumination which was weak at longer wavelengths. This made the red element of images appear faint and caused colors to lose their clarity.

CoolLED pE-100wht light source and control pod
CoolLED pE-100wht light source and control pod

Features and Benefits

Feature Benefit
Spectral performance matched to that of 100W halogen Familiar images
No variation in color across intensity range No need to color balance
Replaces 100W halogen lamp housing on most current and older microscopes Quick and easy exchange
No unwanted UV Reduced risk of eye damage
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use No need to align
Stable & repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 50,000 hours of operating time

pE-100

The pE-100 series is a mercury-free family of compact and simple to use LED illumination systems which can be configured to deliver light directly to a microscope, or via a liquid light guide or multimode fiber. Systems can be specified at any one of 20 different LED wavelengths.

Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%. Remote control is available via a TTL trigger.

The system has been developed for applications requiring a single LED excitation wavelength. It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise intensity control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location. The user can select from 20 different LED wavelengths, ranging from the near-UV at 365nm to the near-IR at 770nm.

There are three standard pE-100 configuration options:

Direct-fit (pE-100) for connecting to a microscope by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide (pE-100light guide) with a fixed 3mm diameter, liquid light guide

Fiber (pE-100fiber) with an SMA connection for accepting multimode fibers. The pE-100fiber has been designed with efficient coupling into a wide range of multimode fibers. The pE-100fiber also includes excitation filter holders.

Features and Benefits

Feature Benefit
Broadest LED spectrum available: 365-770nm Replaces metal halide or mercury
Compatible with existing single and multi-band filter sets No need to buy new filters
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use no alignment, a once‐only adjustment
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time

Control Options

Manual Control Instant On/Off
  Intensity control in 1% steps from 0 to 100%
Remote On/Off via single TTL through BNC on control pod
  Triggering speed <20 µs

Key Benefits

  • Combines software and hardware timing to flexibly match illumination to camera exposure and reduce photo damage
  • Standalone front panel control, works with any software
  • LED indicators allow researcher to keep track of experiment
  • Streaming protocols can be changed on-the-fly in response to experimental needs
  • Supports virtual global shutter modes on scientific CMOS cameras

Need a product demo? Contact us.