CoolLED Light Sources

BioVision Technologies offers the following microscope illumination products from CoolLED Ltd.:

CoolLED Logo

pE-4000

The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy. The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, giving it the broadest spectrum of illumination available.

For consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system.

For total control over the excitation source to improve imaging, the pE-4000 “Advanced” mode provides selection and control of individual wavelengths settings.

Features and Benefits

Feature Benefit
Broadest LED spectrum available: 365-770nm Matches LED peaks to existing microscope filters
Four channel wavelength-grouping design Matches with all commercially available dual, triple, and quad filters
Individual control of selected LED wavelengths Less background, no filter wheel or shutters
Integrated with many imaging software packages Can be incorporated into all experimental parameters
Instant on/off No shutters required, no warm up or cool down
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time

Features for Multi-User Facilities and Core Facilities

Feature Benefit
Feature Benefit
Presets Lab manager can match white spectrum to existing filter cubes
Higher‐contrast images From matched white spectrum
Simple installation and use  
No training required  

Features for Advanced Research

Feature Benefit
Feature Benefit
Individual LED wavelength selection Matches LED peaks to existing microscope filters
Rapid switching between LED wavelengths Enables capture of high‐speed events
TTL and USB interfaces Flexible integration with imaging software
Allows installation of excitation filters in optical path Provides controlled switching with no moving parts
Analog voltage inputs for intensity control High-speed switching
Optical Feedback Provides for applications requiring higher stability
Internal function generator For electophysiology and optogenetics applications
CoolLED pE-4000 light source and control pod
CoolLED pE-4000 light source and control pod

pE-300white

CoolLED’s pE-300white offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains. The pE-300white Light Source can be fitted directly to the microscope or specified with a liquid light guide. The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators. The system is perfect for hospital and regular research laboratory environments.

The pE-300white is a compact and simple to use LED illumination unit. It provides a broad spectrum of illumination, covering the excitation bands of common fluorescent stains. Manual operation is by a remote control pod with instant on/off and intensity control from 0 to 100 percent. Remote control is available via USB or TTL trigger.

Features and Benefits

Feature Benefit
Feature Benefit
Clean bright illumination across the spectrum Excites common fluorescent stains
Order for existing single and multi-band filter sets No need to buy new filters
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use No alignment, a once only adjustment
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time
CoolLED pE-300white light source and control pod
CoolLED pE-300white light source and control pod

pE-100wht

The pE-100wht is a powerful white LED illumination system designed to replace a 100W halogen lamp. Histologists and cytologists who are familiar with results from a conventional halogen lamp can immediately make accurate and reliable diagnosis using the pE-100wht as colors will appear the same. Unlike that of a Halogen lamp, the color balance of the pE-100wht illumination system does not vary with intensity, removing the need to make any adjustment. The many additional benefits which LEDs offer—mercury-free operation, instant on/off, control and repeatability—mean that laboratories using halogen lamps will find that the move to the pE-100wht is obvious and simple.

The pE-100wht is a compact and simple-to-use LED illumination system for transmitted light applications and comprises a pE-100wht LED light source, control pod, and power supply. The user is given instant on/off and intensity control in one‐percent steps from the manual control pod which can be positioned close at hand. Remote control is available via a TTL trigger.

The system uses a new LED technology which has a spectrum closely matching halogen. Previous LED technology offered illumination which was weak at longer wavelengths. This made the red element of images appear faint and caused colors to lose their clarity.

CoolLED pE-100wht light source and control pod
CoolLED pE-100wht light source and control pod

Features and Benefits

Feature Benefit
Spectral performance matched to that of 100W halogen Familiar images
No variation in color across intensity range No need to color balance
Replaces 100W halogen lamp housing on most current and older microscopes Quick and easy exchange
No unwanted UV Reduced risk of eye damage
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use No need to align
Stable & repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 50,000 hours of operating time

pE-100

The pE-100 series is a mercury-free family of compact and simple to use LED illumination systems which can be configured to deliver light directly to a microscope, or via a liquid light guide or multimode fiber. Systems can be specified at any one of 20 different LED wavelengths.

Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%. Remote control is available via a TTL trigger.

The system has been developed for applications requiring a single LED excitation wavelength. It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise intensity control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location. The user can select from 20 different LED wavelengths, ranging from the near-UV at 365nm to the near-IR at 770nm.

CoolLED pE-100 light source and control panel

CoolLED pE-100 light source and control pod

Brochure in PDF format

There are three standard pE-100 configuration options:

Direct-fit (pE-100) for connecting to a microscope by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide (pE-100light guide) with a fixed 3mm diameter, liquid light guide

Fiber (pE-100fiber) with an SMA connection for accepting multimode fibers. The pE-100fiber has been designed with efficient coupling into a wide range of multimode fibers. The pE-100fiber also includes excitation filter holders.

Features and Benefits

Feature Benefit
Broadest LED spectrum available: 365-770nm Replaces metal halide or mercury
Compatible with existing single and multi-band filter sets No need to buy new filters
Instant on/off No shutters required, no warm up or cool down
Simple to fit, simple to use no alignment, a once‐only adjustment
Stable and repeatable Reliable and consistent results
Wide range of microscope adaptors Fits most microscopes
Precise intensity control in 1% steps (0 to 100%) No ND filters required
Excellent uniformity over field of view Fixed and stable, no alignment necessary
Long lifetime Expected to exceed 25,000 hours of operating time

Control Options

Manual Control Instant On/Off
  Intensity control in 1% steps from 0 to 100%
Remote On/Off via single TTL through BNC on control pod
  Triggering speed <20 µs

Key Benefits

  • Combines software and hardware timing to flexibly match illumination to camera exposure and reduce photo damage
  • Standalone front panel control, works with any software
  • LED indicators allow researcher to keep track of experiment
  • Streaming protocols can be changed on-the-fly in response to experimental needs
  • Supports virtual global shutter modes on scientific CMOS cameras

Need a product demo? Contact us.