Diskovery

Multi-modal imaging platfrom from Spectral Applied Research and Andor

The Diskovery platform brings together ultimate flexibility and performance. It natively includes Borealis illumination and seamlessly combines many imaging modalities:

  • Spinning disk confocal
  • Zoomable widefield
  • TIRF
  • Super resolution
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The Platform for Advanced Spinning Disk Confocal Microscopy

The Diskovery Platform is built to compete with any spinning disk confocal imager available, and designed to support the imaging needs of today's research. It makes optimal use of modern cameras and laser light sources, and employs advanced proprietary technology to increase light output. It can be added to existing microscopes or purchased with an integrated microscopy system. It is designed for in‐the‐field upgrades to add capability as requirements change.

The Diskovery is suited to fluorescence work, super resolution microscopy and work with large specimens, TIRF imaging, single‐molecule work, single particle tracking, FRET, quantitative FRAP, multi‐wavelength photoactivation, and super resolution imaging.

To provide for both widefield and confocal imaging, the Diskovery Platform can move its disk in and out of the optical path under software control, allowing for mixed‐mode work.

The needs for highest-sensitivity acquisition, the highest speed, and the largest field of view call for cameras with distinct pixel sizes and extents of field of view. The Diskovery Platform addresses all of these: It can match excitation light to the camera or the application; Its disks support as many as three pinhole sizes, providing the best performance with the best EMCCD cameras and scientific CMOS cameras; Disks can be removed and others installed without returning the Diskovery Platform to the factory, allowing reconfiguration to suit varied needs.

Borealis WF technology allows the Diskovery Platform to adjust the field of excitation to match the full frame field of view of the camera without wasting laser output. It also provides for limiting excitation to smaller fields of view. Since power at the sample plane increases as the area of the field of excitation decreases, one can trade field of excitation for excitation power.

Proprietary technology allows the Diskovery Platform to produce light characterized by brightness and uniformity that only Spectral Applied Research delivers. Patented Borealis technology allows the use of multiple lasers without an excitation dichroic, allowing the Diskovery to image all the pinholes of the disk in a common optical plane. This is a distinct benefit for simultaneous or sequential TIRF imaging of multiple wavelengths.

The Diskovery is available configured for multiple identical cameras or for disparate cameras, as well as for a single camera. BioVision Technologies will equip it to match your needs, be they highest‐speed multiple‐wavelength work, multiple‐camera flexibility for multiple imaging needs, or the most practical performance for single‐camera imaging. We offer it for use with your microscope stand or for use with our Aries integrated microscope, for use with leading imaging software including MetaMorph and MetaFluor.

For more information about the Diskovery Platform, please contact your local BioVision Technologies representative.

Specifications

Specification Detail
Usable lasers 405 nm, 445 nm, 473 nm, 488 nm, 491 nm, 514 nm, 532 nm, 552 nm, 561 nm, 593 nm, 630 nm, 640 nm, 730 nm, 780 nm
Usable laser powers 1mW to 3 W with Spectral Applied Research laser system
Brightness controls Solid state, ND wheel, and/or AOTF and FLCR™
Laser introduction Fiber method (FC connector)
Safety Integrated interlock
Number of lines Spectral Applied Research laser systems support as many as seven lasers with computer control
Laser software support MetaMorph, MetaFluor, Micro-Manager, MATLAB, HC Image, Volocity, Explora Nova, TTL; Spectral LMM GUI and SDK available
Compatible microscope Any microscope with a dedicated 100% video port
Objectives Wide range of fluorescence‐grade objectives; TIRF support requires objective with N.A. of 1.4 or better
Mode changes Supports methods for simultaneous FRAP, for Epi‐fluorescence, for super resolution imaging
Camera options 1‒4 integrated ports for simultaneous imaging, more with image splitters or dual‐chip cameras such as Hamamatsu Orca‐D2 x(C11254‐10B)
Emission filter Supports 1‒2 motorized filter wheels and/or single manual filter paddles
Excitation dichroic Not required
Camera splitters Reflecting >500nm, 565nm, 605nm, 640nm, 750nm mounted in custom alignment holders
Camera registration Perfect pixel–pixel registration with X‐Y translation, rotation and axial focus correcting for magnification and chromatic shifts
Camera support Custom mounts for most camera types and manufactures
Camera magnification Custom‐matched to meet the resolution for your experiments
Modes TIRF, Spinning Disk, Super Resolution Localization Microscope, Widefield
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